Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Activated zinc transporter ZIP7 as an indicator of anti-hormone resistance in breast cancer

Ziliotto, Silvia, Gee, Julia M. W., Ellis, Ian O., Green, Andrew R., Finlay, Pauline, Gobbato, Anna and Taylor, Kathryn M. 2019. Activated zinc transporter ZIP7 as an indicator of anti-hormone resistance in breast cancer. Metallomics 11 (9) , pp. 1579-1592. 10.1039/C9MT00136K

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (5MB) | Preview

Abstract

ZIP7, a member of the ZIP family of zinc importers, resides on the endoplasmic reticulum membrane and transports zinc from intracellular stores to the cytoplasm after activation by CK2 phosphorylation on two serine residues (S275 and S276). ZIP7 is known to be required for the growth of anti-hormone resistant breast cancer models, especially those with acquired tamoxifen resistance developed from MCF-7. Using our new pS275S276ZIP7 antibody which only recognises activated ZIP7 (pZIP7), we have demonstrated that the hyperactivation of ZIP7 is prevalent in tamoxifen-resistant breast cancer cells. This evidence suggests that pZIP7 might have potential as a biomarker of acquired resistance to such anti-hormones in breast cancer, a current unmet clinical need. In this regard, we have also developed a new immunohistochemical assay for pZIP7 which allowed pZIP7 to be tested on a small clinical series of breast cancer tissues confirming its prevalence in such tumours and relationship to a variety of clinicopathological parameters and biomarkers previously associated with endocrine resistant phenotypes, notably increased activated MAPK signalling, expression of ErbB2, CD71 and the proto-oncogene c-Fos, as well as with increased tumour grade.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Publisher: Royal Society of Chemistry
ISSN: 1756-5901
Funders: Wellcome Trust, Tenovus Cancer Care
Date of First Compliant Deposit: 10 September 2019
Date of Acceptance: 25 July 2019
Last Modified: 14 Nov 2019 14:42
URI: http://orca.cf.ac.uk/id/eprint/125375

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics