The use of intracellular dialysis to study signal transduction coupling in the squid giant axon

Allen, T.J.A. and Knight, D.E. 1992. The use of intracellular dialysis to study signal transduction coupling in the squid giant axon. Journal of Neuroscience Methods 42 (3) , pp. 169-174. 10.1016/0165-0270(92)90096-V

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Abstract

The squid giant axon has proved a useful model in the study of ionic channel gating, intracellular homeostasis and receptor-mediated signal transduction leading to generation of intracellular second messengers. In the latter category, previous studies on activation of adenylate or guanylate cyclase have used intact and intracellularly perfused axons to investigate the effects of extra- and intracellular agents on the transduction processes. However, the perfusion of the axon interior washes out many factors which may be important in the processes under study. We introduce here the use of porous cellulose dialysis tubing as a means to circumvent these problems. We find that this dialysis technique is a simple procedure to set-up, and the serotonin/G-protein/adenylate cyclase system can readily be studied in the dialysed axon. This approach should allow investigation under conditions which retain asymmetric transmembrane conditions.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Elsevier
ISSN:	0165-0270
Date of Acceptance:	14 February 1992
Last Modified:	15 Jan 2020 12:15
URI:	https://orca.cardiff.ac.uk/id/eprint/127186

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