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Characterization of bacteria by multiparameter flow cytometry

Allman, R, Hann, A C, Manchee, R and Lloyd, D 2008. Characterization of bacteria by multiparameter flow cytometry. JOURNAL OF APPLIED BACTERIOLOGY 73 (5) , 438--444. 10.1111/j.1365-2672.1992.tb05001.x

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Abstract

An arc-lamp based flow cytometer was used to obtain high resolution measurements of the light scattering characteristics and DNA contents of eight different bacteria. Light scatter profiles of bacteria are a useful first step when flow cytometry is used to characterize organisms. Scanning and transmission electron microscopy of the bacterial samples demonstrate that the structural basis of the light scattering profiles is not always clear, i.e. some organisms appear to have anomalous light scattering characteristics. The use of a third measurement parameter, DNA content, allowed much better discrimination of the organisms. Flow cytometry shows great promise as a method for the rapid discrimination and identification of bacterial populations.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
ISSN: 0021-8847
Date of Acceptance: 6 July 1992
Last Modified: 13 Feb 2020 17:00
URI: http://orca.cf.ac.uk/id/eprint/127693

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