Lipids and lipid metabolism in the microaerobic free-living diplomonad Hexamita sp.

Biagini, Giancarlo A, Rutter, Andrew J, Finlay, Bland J and Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571 1998. Lipids and lipid metabolism in the microaerobic free-living diplomonad Hexamita sp. European Journal of Protistology 34 (2) , 148--152. 10.1016/S0932-4739(98)80025-4

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Abstract

Hexamita sp. is a free-living heterotrophic flagellate ubiquitous in microaerobic environments. It is one of the deepest branching extant eukaryotes, lacking mitochondria and probably an energy-yielding oxidative metabolism. Hexamita is cultured in a medium supplemented with foetal calf serum, a ready supply of lipids essential for growth. This study has shown that Hexamita does not depend entirely on exogenous lipid supplies and is able to incorporate [1-14C] acetate, albeit poorly, into its fatty acids, phospholipids and sterols. The major polar lipids detected were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, sphingomyelin and cerebrosides. Triacylglycerols were identified as the main storage lipids together with sterol esters. The major lipids identified in Hexamita reflect the lipids detected in the culture medium (with the exception of phosphatidylethanolamine); these lipids are believed to have been sequestered directly from the culture medium. It is possible that phosphatidylethanolamine was synthesised by Hexamita via phosphotransferase pathways. Incubation with radiolabeled palmitate, oleate or stéarate showed that no further modifications (e.g. desaturation or elongation) had taken place.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Elsevier
ISSN:	0932-4739
Last Modified:	26 Oct 2022 08:32
URI:	https://orca.cardiff.ac.uk/id/eprint/127713

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