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Enzyme activity in organic solvent as a function of water activity determined by membrane inlet mass spectrometry

Degn, Hans and Lloyd, David 1992. Enzyme activity in organic solvent as a function of water activity determined by membrane inlet mass spectrometry. Biotechnology Techniques 6 (2) , pp. 161-164. 10.1007/BF02438824

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Abstract

Membrane inlet mass spectrometry (MIMS) is introduced as a method for measuring water activity in nonpolar solvents, aqueous solutions and gas phase. The determination of the rate of hydrolysis of diphenyl carbonate by porcine liver esterase as a function of water activity in diisopropyl ether is presented as an example. A linear relationship is found between the enzyme activity and the water activity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Springer Verlag (Germany)
ISSN: 0951-208X
Last Modified: 05 Mar 2020 15:30
URI: http://orca.cf.ac.uk/id/eprint/127772

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