Avoid excessive oxygen levels in experiments with organisms, tissues and cells.

Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571 and Williams, Catrin F. ORCID: https://orcid.org/0000-0001-8619-2581 2015. Avoid excessive oxygen levels in experiments with organisms, tissues and cells. Advances in Microbial Physiology 67 , 293--314. 10.1016/bs.ampbs.2015.09.001

Full text not available from this repository.

Abstract

O2 levels encountered in vivo in cells and tissues are almost always at least an order of magnitude less than atmospheric pO2 because of sensing, signalling and bioenergetic demand. Although deleterious reactions are minimized by protective mechanisms (residual toxic products scavenged and detoxified) ambient levels should be mimicked in experiments with whole organisms, their isolated organs, tissues or cells and also with cultures of cell lines. These are also important issues for microorganisms inhabiting low O2 niches within higher organisms and their cells. Here, we highlight the importance of optimization of micro-aerobic conditions for experimentation and the deleterious consequences of not doing so.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Engineering Biosciences
Publisher:	Academic Press Inc.
ISSN:	0065-2911
Last Modified:	25 Nov 2022 12:07
URI:	https://orca.cardiff.ac.uk/id/eprint/127832

Citation Data

Cited 7 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item