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Use of two oxonols and a fluorescent tetrazolium dye to monitor starvation of Escherichia coli in seawater by flow cytometry

Lopez-Amoros, R, Mason, DJ and Lloyd, D 2000. Use of two oxonols and a fluorescent tetrazolium dye to monitor starvation of Escherichia coli in seawater by flow cytometry. Journal of Microbiological Methods 22 (2) , 165--176. 10.1016/0167-7012(94)00073-G

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Abstract

Flow cytometry was used to study starvation of Escherichia coli in artificial seawater using two membrane potential sensitive dyes and redox dye. The oxonols, DiBAC4(3) and oxonol VI, enabled assessment of energization states. Parallel use of these two dyes suggests that oxonol VI is the more useful for detection of membrane depolarization. The NADPH reducibility of 5-cyano-2,3-ditolyl tetrazolium chloride can be used to estimate loss of membrane integrity during survival in seawater.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0167-7012
Date of Acceptance: 10 October 1994
Last Modified: 10 Mar 2020 10:45
URI: http://orca.cf.ac.uk/id/eprint/127842

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