Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria.

Mason, D J, Lopez-Amoros, R, Allman, R, Stark, J M and Lloyd, D 1995. The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria. JOURNAL OF APPLIED BACTERIOLOGY 78 (3) , 309--315. 10.1111/j.1365-2672.1995.tb05031.x

Full text not available from this repository.

Abstract

Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
ISSN: 0021-8847
Date of Acceptance: 4 November 1994
Last Modified: 09 Mar 2020 16:15
URI: http://orca.cf.ac.uk/id/eprint/127853

Citation Data

Cited 102 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item