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The preparation of rat‐liver mitochondria by conventional differential centrifugation and by zonal Centrifugation, after various disruption procedures: A comparative study

Klucis, E., Lloyd, D. and Roach, G.I. 1968. The preparation of rat‐liver mitochondria by conventional differential centrifugation and by zonal Centrifugation, after various disruption procedures: A comparative study. Biotechnology and Bioengineering 10 (3) , pp. 321-330. 10.1002/bit.260100307

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Abstract

Rat liver was subjected to three different, disruption procedures (homogenization, explosive decompression, and Chaikoff press) and mitochondria were subsequently isolated by conventional differential Centrifugation and by zonal Centrifugation. The properties of these mitochondria were investigated by polarographic measurement of oxygen uptake and they were examined by electron microscopy. All three methods of disruption gave mitochondria which showed respiratory control. Nitrogen cavitation gave the most reproducible conditions for cell breakage and zonal Centrifugation gave good separation of subcellular organdies in extracts produced by this method. Some separation of the heterogenous mitochondrial populations was achieved by zonal Centrifugation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Wiley
ISSN: 0006-3592
Last Modified: 02 Mar 2020 11:45
URI: http://orca.cf.ac.uk/id/eprint/127905

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