Antibodies against phospholipase C inhibit smooth muscle contraction induced by acetylcholine and histamine

Moraru, Ion I., Popescu, Laurentziu M., Vidulescu, Cristina and Tzigaret, Cezar 1987. Antibodies against phospholipase C inhibit smooth muscle contraction induced by acetylcholine and histamine. European Journal of Pharmacology 138 (3) , pp. 427-431. 10.1016/0014-2999(87)90483-3

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Abstract

The rationale of this study was to obtain a highly specific inhibitor of phospholipase C by raising rabbit antibodies against the purified bacterial phospholipase C. The antibodies inhibited the enzyme activity in vitro and, as shown by immunofluorescence, cross-reacted with the membrane-bound phospholipase C of isolated guinea-pig smooth muscle cells. Incubation (0–4 h) of guinea-pig taenia coli and ileum with antibodies resulted in a progressive inhibition (up to 85%) of the contractile response evoked by 2 μM acetylcholine or 2 μM histamine but did not inhibit significantly the contraction produced by prostaglandin F2α (0.1 μM). These inhibitory antibodies presumably represent the ‘missing tool’ needed to establish unequivocally if a given agonist acts via stimulation of the membrane-bound phospholipase C, and implicity phosphoinositide hydrolysis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Neuroscience and Mental Health Research Institute (NMHRI)
Publisher:	Elsevier
ISSN:	0014-2999
Date of Acceptance:	28 April 1987
Last Modified:	24 Jan 2020 10:45
URI:	https://orca.cardiff.ac.uk/id/eprint/128534

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