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Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc

Bowen, Benjamin J., McGarrity, Adam R., Szeto, Jenn-Yeu A., Pudney, Christopher R. and Jones, D. Dafydd 2020. Switching protein metalloporphyrin binding specificity by design from iron to fluorogenic zinc. Chemical Communications 56 (31) , pp. 4308-4311. 10.1039/D0CC00596G
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Abstract

Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b562 from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Royal Society of Chemistry
ISSN: 1359-7345
Date of First Compliant Deposit: 12 March 2020
Date of Acceptance: 12 March 2020
Last Modified: 31 Jul 2020 15:19
URI: http://orca.cf.ac.uk/id/eprint/130390

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