Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Tetraspanin CD53 promotes lymphocyte eecirculation by stabilizing L-selectin surface expression

Demaria, Maria C., Yeung, Louisa, Peeters, Rens, Wee, Janet L., Mihaljcic, Masa, Jones, Eleanor L., Nasa, Zeyad, Alderuccio, Frank, Hall, Pamela, Smith, Brodie C., Binger, Katrina J., Hammerling, Gunther, Kwok, Hang Fai, Newman, Andrew, Ager, Ann, van Spriel, Annemiek, Hickey, Michael J. and Wright, Mark D. 2020. Tetraspanin CD53 promotes lymphocyte eecirculation by stabilizing L-selectin surface expression. iScience 23 (5) , 101104. 10.1016/j.isci.2020.101104

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (3MB) | Preview

Abstract

Tetraspanins regulate key processes in immune cells; however, the function of the leukocyte-restricted tetraspanin CD53 is unknown. Here we show that CD53 is essential for lymphocyte recirculation. Lymph nodes of Cd53−/− mice were smaller than those of wild-type mice due to a marked reduction in B cells and a 50% decrease in T cells. This reduced cellularity reflected an inability of Cd53−/− B and T cells to efficiently home to lymph nodes, due to the near absence of L-selectin from Cd53−/− B cells and reduced stability of L-selectin on Cd53−/− T cells. Further analyses, including on human lymphocytes, showed that CD53 stabilizes L-selectin surface expression and may restrain L-selectin shedding via both ADAM17-dependent and ADAM17-independent mechanisms. The disruption in lymphocyte recirculation in Cd53−/− mice led to impaired immune responses dependent on antigen delivery to lymph nodes. Together these findings demonstrate an essential role for CD53 in lymphocyte trafficking and immunity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
ISSN: 2589-0042
Date of First Compliant Deposit: 10 June 2020
Date of Acceptance: 23 April 2020
Last Modified: 11 Jun 2020 12:00
URI: http://orca.cf.ac.uk/id/eprint/132308

Citation Data

Cited 3 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics