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Establishment of long-term ostracod epidermal culture

Morgan, Sian R., Paletto, Laura, Rumney, Benjamin, Malik, Farhana T., White, Nick, Lewis, Philip N., Parker, Andrew R., Holden, Simon, Meek, Keith M. and Albon, Julie 2020. Establishment of long-term ostracod epidermal culture. In Vitro Cellular and Developmental Biology - Animal 56 (9) , pp. 760-772. 10.1007/s11626-020-00508-8

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Abstract

Primary crustacean cell culture was introduced in the 1960s, but to date limited cell lines have been established. Skogsbergia lerneri is a myodocopid ostracod, which has a body enclosed within a thin, durable, transparent bivalved carapace, through which the eye can see. The epidermal layer lines the inner surface of the carapace and is responsible for carapace synthesis. The purpose of the present study was to develop an in vitro epidermal tissue and cell culture method for S. lerneri. First, an optimal environment for the viability of this epidermal tissue was ascertained, while maintaining its cell proliferative capacity. Next, a microdissection technique to remove the epidermal layer for explant culture was established and finally, a cell dissociation method for epidermal cell culture was determined. Maintenance of sterility, cell viability and proliferation were key throughout these processes. This novel approach for viable S. lerneri epidermal tissue and cell culture augments our understanding of crustacean cell biology and the complex biosynthesis of the ostracod carapace. In addition, these techniques have great potential in the fields of biomaterial manufacture, the military and fisheries, for example, in vitro toxicity testing.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Additional Information: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Publisher: Springer Verlag (Germany) / Society for In-Vitro Biology
ISSN: 1071-2690
Funders: DSTL
Date of First Compliant Deposit: 12 November 2020
Date of Acceptance: 9 September 2020
Last Modified: 13 Nov 2020 15:30
URI: http://orca.cf.ac.uk/id/eprint/136312

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