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Lipid profiling of mouse intestinal organoids for studying APC mutations

Jukes, Zoe, Freier, Anne, Glymenaki, Maria, Brown, Richard, Parry, Lee, Want, Elizabeth, Vorkas, Panagiotis A. and Li, Jia V. 2021. Lipid profiling of mouse intestinal organoids for studying APC mutations. Bioscience Reports 10.1042/BSR20202915

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Abstract

RESEARCH ARTICLE| FEBRUARY 23 2021 Lipid Profiling of Mouse Intestinal Organoids for studying APC Mutations Zoë Jukes ; Anne Freier ; Maria Glymenaki ; Richard Brown ; Lee Parry ; Elizabeth Want ; Panagiotis A Vorkas ; Jia V. Li Crossmark: Check for Updates Biosci Rep (2021) BSR20202915. https://doi.org/10.1042/BSR20202915 Article history Split-Screen PDF Link PDF Share Icon Share Cite Icon Cite Get Permissions Inactivating mutations including both germline and somatic mutations in the adenomatous polyposis coli (APC) gene drives most familial and sporadic colorectal cancers. Understanding the metabolic implications of this mutation will aid to establish its wider impact on cellular behaviour and potentially inform clinical decisions. However, to date, alterations in lipid metabolism induced by APC mutations remain unclear. Intestinal organoids have gained widespread popularity in studying colorectal cancer and chemotherapies, because their three-dimensional structure more accurately mimics an in vivo environment. Here, we aimed to investigate intra-cellular lipid disturbances induced by APC gene mutations in intestinal organoids using a reversed-phase ultra-high-performance liquid chromatography mass spectrometry (RP-UHPLC-MS)-based lipid profiling method. Lipids of the organoids grown from either wildtype (WT) or mice with Apc mutations (Lgr5–EGFP-IRES-CreERT2Apcfl/fl) were extracted and analysed using RP-UHPLC-MS. Concentrations of phospholipids (e.g. PC(16:0/16:0), PC(18:1/20:0), PC(38:0), PC(18:1/22:1)), ceramides (e.g. Cer(d18:0/22:0), Cer(d42:0), Cer(d18:1/24:1)) and hexosylceramide (e.g. HexCer(d18:1/16:0), HexCer(d18:1/22:0)) were higher in Apcfl/fl organoids, whereas levels of sphingomyelins (e.g. SM(d18:1/14:0), SM(d18:1/16:0) ) were lower compared to WT. These observations indicate that cellular metabolism of sphingomyelin was upregulated, resulting in the cellular accumulation of ceramides and production of HexCer due to the absence of Apcfl/fl in the organoids. Our observations demonstrated lipid profiling of organoids and provided an enhanced insight into the effects of the APC mutations on lipid metabolism, making for a valuable addition to screening options of the organoid lipidome.

Item Type: Article
Date Type: Published Online
Status: In Press
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Publisher: Portland Press
ISSN: 0144-8463
Date of First Compliant Deposit: 5 January 2021
Date of Acceptance: 5 January 2021
Last Modified: 24 Feb 2021 13:29
URI: http://orca.cf.ac.uk/id/eprint/137323

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