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Simultaneous aerosol and intramuscular immunization with influenza vaccine induces powerful protective local T cell and systemic antibody immune responses in pigs

Martini, Veronica, Paudyal, Basu, Chrun, Tiphany, McNee, Adam, Edmans, Matthew, Atangana Maze, Emmanuel, Clark, Beckie, Nunez, Alejandro, Dolton, Garry, Sewell, Andrew, Beverley, Peter, MacLoughlin, Ronan, Townsend, Alain and Tchilian, Elma 2021. Simultaneous aerosol and intramuscular immunization with influenza vaccine induces powerful protective local T cell and systemic antibody immune responses in pigs. Journal of Immunology 206 (2) , ji2001086. 10.4049/jimmunol.2001086

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Abstract

A vaccine providing both powerful Ab and cross-reactive T cell immune responses against influenza viruses would be beneficial for both humans and pigs. In this study, we evaluated i.m., aerosol (Aer), and simultaneous systemic and respiratory immunization (SIM) by both routes in Babraham pigs, using the single cycle candidate influenza vaccine S-FLU. After prime and boost immunization, pigs were challenged with H1N1pdm09 virus. i.m.-immunized pigs generated a high titer of neutralizing Abs but poor T cell responses, whereas Aer induced powerful respiratory tract T cell responses but a low titer of Abs. SIM pigs combined high Ab titers and strong local T cell responses. SIM showed the most complete suppression of virus shedding and the greatest improvement in pathology. We conclude that SIM regimes for immunization against respiratory pathogens warrant further study.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Additional Information: This article is distributed under the terms of the CC BY 4.0 Unported license
Publisher: American Association of Immunologists
ISSN: 0022-1767
Date of First Compliant Deposit: 6 January 2021
Date of Acceptance: 18 November 2020
Last Modified: 08 Jan 2021 15:37
URI: http://orca.cf.ac.uk/id/eprint/137354

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