A genome guided evaluation of the Lab4 probiotic consortium

Baker, L.M., Webberley, T.S., Masetti, G., Hughes, T.R. ORCID: https://orcid.org/0000-0003-2348-3490, Marchesi, J.R., Jack, A.A., Joyce, T.S.C., Allen, M.D., Plummer, S.F., Michael, D.R., Ramanathan, G., Del Sol, R. and Facey, P.D. 2021. A genome guided evaluation of the Lab4 probiotic consortium. Genomics 113 (6) , pp. 4028-4038. 10.1016/j.ygeno.2021.08.007

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Abstract

Draft genome sequences of the Lab4 probiotic consortium were deposited in Genbank: Bifidobacterium animalis subsp lactis CUL34 (PRJNA482550), Bifidobacterium bifidum CUL20 (PRJNA559984), Lactobacillus acidophilus CUL60 (PRJNA482335), Lactobacillus acidophilus CUL21 (PRJNA482434). Probiogenomic analyses confirmed existing taxonomies and identified putative gene sequences that were functionally related to the performance of each organism during in vitro assessments of bile and acid tolerability, adherence to enterocytes and susceptibility to antibiotics. Genomic stability predictions identified no significant risk of gene acquisition of both antibiotic resistance and virulence genes. These observations were supported by acute phase and repeat dose tolerability studies in Wistar rats. High doses of Lab4 did not result in mortalities, clinical/histopathological abnormalities nor systemic toxicity. Increased faecal numbers of Lab4 in supplemented rats implied survival through the gastrointestinal tract and/or impact the intestinal microbiota composition. In summary, this study provides multifaceted support for probiotic functionality and the safety of the Lab4 consortium.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Elsevier
ISSN:	0888-7543
Date of First Compliant Deposit:	9 February 2022
Date of Acceptance:	10 August 2021
Last Modified:	23 May 2023 21:05
URI:	https://orca.cardiff.ac.uk/id/eprint/147355

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