Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Autoradiographic distribution of [3H]l-NG-Nitro-arginine binding in rat brain

Kidd, Emma Jane, Michel, A. D. and Humphrey, P. P. A. 1995. Autoradiographic distribution of [3H]l-NG-Nitro-arginine binding in rat brain. Neuropharmacology 34 (1) , pp. 63-73. 10.1016/0028-3908(94)00132-C

Full text not available from this repository.

Abstract

The distribution of nitric oxide synthase (NOS), the enzyme which produces nitric oxide, has previously been studied in the rat central nervous system (CNS) using the NADPH-diaphorase technique and anti-NOS antibodies. However, the former method may not always be selective for NOS while the latter is not quantitative. Therefore a selective, quantifiable method would be desirable. l-NG-Nitro-arginine, an inhibitor of NOS, is available in a tritiated form which we have shown to bind to NOS. We have now examined the regional distribution of [3H]l-NG-nitro-argininebinding in the rat CNS using autoradiography. [3H]l-NG-nitro-arginine specific binding was seen in a number of brain regions with the highest levels in the accessory olfactory bulb, the amygdaloid complex, the Islands of Calleja and the cerebellum. This regional distribution of [3H]l-NG-nitro-argininebinding sites in the rat CNS was, in general, similar to that seen with the NADPH-diaphorase method and anti-NOS antibodies, consistent with the view that all three methods identify NOS in the CNS. Thus, [3H]l-NG-nitro-arginine appears to be a useful radioligand for studying the distribution of NOS in the CNS as its binding is quantifiable and apparently selective for NOS.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords: Nitric oxide synthase; rat central nervous system; [3H]l-NG-nitro-arginine; autoradiography; regional distribution; quantification
Publisher: Elsevier
ISSN: 0028-3908
Last Modified: 03 May 2019 07:11
URI: http://orca.cf.ac.uk/id/eprint/14766

Citation Data

Cited 49 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item