Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome

Knight, Rob ORCID: https://orcid.org/0000-0001-9927-6354, Board-Davies, Emma, Brown, Helen, Clayton, Aled ORCID: https://orcid.org/0000-0002-3087-9226, Davis, Terence ORCID: https://orcid.org/0000-0003-2780-0262, Karatas, Ben, Burston, James, Tabi, Zsuzsanna, Falcon-Perez, Juan M, Paisey, Stephen ORCID: https://orcid.org/0000-0002-2274-3708 and Stephens, Phil ORCID: https://orcid.org/0000-0002-0840-4996 2022. Oral progenitor cell line-derived small extracellular vesicles as a treatment for preferential wound healing outcome. Stem Cells Translational Medicine 11 (8) , pp. 861-875. 10.1093/stcltm/szac037

[thumbnail of finalszac037.pdf]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (76MB) | Preview

Abstract

Scar formation during wound repair can be devastating for affected individuals. Our group previously documented the therapeutic potential of novel progenitor cell populations from the non-scarring buccal mucosa. These Oral Mucosa Lamina Propria-Progenitor Cells (OMLP-PCs) are multipotent, immunosuppressive, and antibacterial. Small extracellular vesicles (sEVs) may play important roles in stem cell–mediated repair in varied settings; hence, we investigated sEVs from this source for wound repair. We created an hTERT immortalized OMLP-PC line (OMLPPCL ) and confrmed retention of morphology, lineage plasticity, surface markers, and functional properties. sEVs isolated from OMLP-PCL were analyzed by nanoparticle tracking analysis, Cryo-EM and fow cytometry. Compared to bone marrow–derived mesenchymal stromal cells (BM-MSC) sEVs, OMLP-PCL sEVs were more potent at driving wound healing functions, including cell proliferation and wound repopulation and downregulated myofbroblast formation. A reduced scarring potential was further demonstrated in a preclinical in vivo model. Manipulation of OMLP-PCL sEVs may provide novel options for non-scarring wound healing in clinical settings.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Medicine
Publisher: Oxford University Press
ISSN: 2157-6564
Funders: Dunhill Medical Trust
Date of First Compliant Deposit: 29 June 2022
Date of Acceptance: 28 April 2022
Last Modified: 05 Jan 2024 03:11
URI: https://orca.cardiff.ac.uk/id/eprint/150847

Citation Data

Cited 1 time in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics