Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Whole-genome microarray analysis identifies up-regulation of Nr4a nuclear receptors in muscle and liver from diet-restricted rats

Oita, Radu C., Mazzatti, D. J., Lim, F. L., Powell, J. R. and Merry, B. J. 2009. Whole-genome microarray analysis identifies up-regulation of Nr4a nuclear receptors in muscle and liver from diet-restricted rats. Mechanisms of Ageing and Development 130 (4) , pp. 240-247. 10.1016/j.mad.2008.12.004

Full text not available from this repository.

Abstract

One of the most conserved methods to significantly increase lifespan in animals is through dietary restriction (DR). The mechanisms by which DR increases survival are controversial but are thought to include improvements in mitochondrial function concomitant with reductions in reactive oxygen species production and alterations in the insulin signalling pathway, resulting in global metabolic adaptation. In order to identify novel genes that may be important for lifespan extension of Brown Norway rats, we compared gene expression profiles from skeletal muscle of 28-month-old animals fed ad libitum or DR diets using whole-genome arrays. Following DR, 426 transcripts were significantly down-regulated whilst only 52 were up-regulated. Included in the up-regulated transcripts were three functionally related previously unidentified DR-regulated genes: Nr4a1, Nr4a2, and Nr4a3. Up-regulation of all three Nr4a receptors was also observed in liver - but not brain - of DR-fed animals. Furthermore, RT-PCR revealed up-regulation of several NR4A transcriptional targets (Ucp-3, Ampk-γ3, Pgc-1α and Pgc-1β) in skeletal muscle of DR animals. Due to the proposed roles of the NR4A nuclear receptors in sensing and responding to changes in the nutritional environment and in regulating glucose and lipid metabolism and insulin sensitivity, we hypothesise that these proteins may contribute to DR-induced metabolic adaptation. © 2009 Elsevier Ireland Ltd. All rights reserved.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Uncontrolled Keywords: dietary restriction; longevity; ageing; nuclear orphan receptor; NR4A; Nur77
Publisher: Elsevier
ISSN: 0047-6374
Last Modified: 19 Mar 2016 22:25
URI: http://orca.cf.ac.uk/id/eprint/16009

Citation Data

Cited 22 times in Google Scholar. View in Google Scholar

Cited 22 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item