Role of enzymic antioxidants in mediating oxidative stress and contrasting wound healing capabilities in oral mucosal/skin fibroblasts and tissues

Lohana, Parkash, Suryaprawira, Albert, Woods, Emma L., Dally, Jordanna, Gait-Carr, Edward, Alaidaroos, Nadia Y.A., Heard, Charles M. ORCID: https://orcid.org/0000-0001-9703-9777, Lee, Kwok Y., Ruge, Fiona, Farrier, Jeremy N., Enoch, Stuart, Caley, Matthew P., Peake, Matthew A., Davies, Lindsay C. ORCID: https://orcid.org/0000-0002-4876-6270, Giles, Peter J. ORCID: https://orcid.org/0000-0003-3143-6854, Thomas, David W. ORCID: https://orcid.org/0000-0001-7319-5820, Stephens, Phil ORCID: https://orcid.org/0000-0002-0840-4996 and Moseley, Ryan ORCID: https://orcid.org/0000-0002-2812-6735 2023. Role of enzymic antioxidants in mediating oxidative stress and contrasting wound healing capabilities in oral mucosal/skin fibroblasts and tissues. Antioxidants 12 (7) , 1374. 10.3390/antiox12071374

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Abstract

Unlike skin, oral mucosal wounds are characterized by rapid healing and minimal scarring, attributable to the “enhanced” healing properties of oral mucosal fibroblasts (OMFs). As oxidative stress is increasingly implicated in regulating wound healing outcomes, this study compared oxidative stress biomarker and enzymic antioxidant profiles between patient-matched oral mucosal/skin tissues and OMFs/skin fibroblasts (SFs) to determine whether superior oral mucosal antioxidant capabilities and reduced oxidative stress contributed to these preferential healing properties. Oral mucosa and skin exhibited similar patterns of oxidative protein damage and lipid peroxidation, localized within the lamina propria/dermis and oral/skin epithelia, respectively. SOD1, SOD2, SOD3 and catalase were primarily localized within epithelial tissues overall. However, SOD3 was also widespread within the lamina propria localized to OMFs, vasculature and the extracellular matrix. OMFs were further identified as being more resistant to reactive oxygen species (ROS) generation and oxidative DNA/protein damage than SFs. Despite histological evaluation suggesting that oral mucosa possessed higher SOD3 expression, this was not fully substantiated for all OMFs examined due to inter-patient donor variability. Such findings suggest that enzymic antioxidants have limited roles in mediating privileged wound healing responses in OMFs, implying that other non-enzymic antioxidants could be involved in protecting OMFs from oxidative stress overall.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Dentistry
Date of First Compliant Deposit:	3 July 2023
Date of Acceptance:	28 June 2023
Last Modified:	01 Dec 2023 15:30
URI:	https://orca.cardiff.ac.uk/id/eprint/160780

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