Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

An ex vivo rodent mandible culture model for bone repair

Smith, Emma, Locke, Matthew, Waddington, Rachel J. and Sloan, Alastair James 2010. An ex vivo rodent mandible culture model for bone repair. Tissue Engineering Part C: Methods 16 (6) , pp. 1287-1296. 10.1089/ten.tec.2009.0698

Full text not available from this repository.

Abstract

To understand fully cellular mechanisms during bone tissue repair and engineering, there is a need to develop reproducible three-dimensional organotypic culture models, whereby cells in their natural extracellular matrix can be manipulated. Limitations in current model systems do not allow for this integrated approach. This study aimed to develop and validate an ex vivo fractured rat mandible model, to investigate specific molecular and cellular processes involved in bone repair. Slices of mandible from 28-day-old male Wistar rats were cultured in Trowel-type cultures at the liquid-gas interface for up to 21 days. Maintenance of cell and tissue architecture and viability was shown within fractured mandible slices during all culture periods. Autoradiographic studies demonstrated that resident cells were actively synthesizing and secreting proteins, and cells of the osteoblast lineage were shown to survive throughout the culture periods. The model was responsive to exogenously added transforming growth factor-β1, with observed increases in cellular migration/proliferation and expression of bone matrix proteins. The ex vivo mandible model developed within this study may represent an ideal system for investigating specific processes of bone repair, as well as a promising alternative to in vivo testing of novel clinical therapeutics. © Copyright 2010, Mary Ann Liebert, Inc.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Publisher: Mary Ann Liebert
ISSN: 1937-3384
Last Modified: 04 Jun 2017 03:03
URI: http://orca.cf.ac.uk/id/eprint/16079

Citation Data

Cited 19 times in Google Scholar. View in Google Scholar

Cited 16 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 10 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item