Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Visualization of fast-moving cells in vivo using digital holographic video microscopy

Sun, H., Song, Bing, Dong, H., Reid, B., Player, M. A., Watson, J. and Zhao, M. 2008. Visualization of fast-moving cells in vivo using digital holographic video microscopy. Journal of biomedical optics 13 (1) , 014007. 10.1117/1.2841050

[img]
Preview
PDF - Published Version
Download (1MB) | Preview

Abstract

Digital in-line holography offers some significant advantages over conventional optical holography and microscopy to image biological specimens. By combining holography with digital video microscopy, an in-line holographic video microscope is developed and is capable of recording spatial 3D holographic images of biological specimens, while preserving the time dimension. The system enables high-speed video recording of fast cell movement, such as the rapid movement of blood cells in the blood stream in vivo. This capability is demonstrated with observations of fast 3-D movement of live cells in suspension cultures in response to a gentle shake to the Petri dish. The experimental and numerical procedures are incorporated with a fast reconstruction algorithm for reconstruction of holographic video frames at various planes (z axis) from the hologram and along the time axis. The current system enables both lateral and longitudinal resolutions down to a few micrometers. Postreconstruction processing of background subtraction is utilized to eliminate noise caused by scattered light, thereby enabling visualization of, for example, blood streams of live Xenopos tadpoles. The combination of digital holography and microscopy offers unique advantages for imaging of fast moving cells and other biological particles in three dimensions in vivo with high spatial and temporal resolution. © 2008 Society of Photo-Optical Instrumentation Engineers.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Additional Information: 9 page article. Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/1083-3668/ (accessed 28/08/2014)
Publisher: Society of Photo-Optical Instrumentation Engineers
ISSN: 1083-3668
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 03:03
URI: http://orca.cf.ac.uk/id/eprint/16087

Citation Data

Cited 18 times in Google Scholar. View in Google Scholar

Cited 28 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics