Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Transglutaminase 2 is needed for the formation of an efficient phagocyte portal in macrophages engulfing apoptotic cells

Toth, Beata, Garabuczi, Eva, Sarang, Zsoly, Vereb, Gyorgy, Vamosi, Gyorgy, Aeschlimann, Daniel, Blasko, Bernadett, Becsi, Baliant, Erdodi, Ferenc, Lacy-Hulbert, Adam, Zhang, Ailiang, Falasca, Laura, Birge, Raymond B., Balajthy, Zoltan, Melino, Gerry, Fesus, Laszlo and Szondy, Zsuzsa 2009. Transglutaminase 2 is needed for the formation of an efficient phagocyte portal in macrophages engulfing apoptotic cells. Journal of Immunology 182 (4) , pp. 2084-2092. 10.4049/jimmunol.0803444

Full text not available from this repository.

Abstract

Transglutaminase 2 (TG2), a protein cross-linking enzyme with many additional biological functions, acts as coreceptor for integrin beta(3). We have previously shown that TG2(-/-) mice develop an age-dependent autoimmunity due to defective in vivo clearance of apoptotic cells. Here we report that TG2 on the cell surface and in guanine nucleotide-bound form promotes phagocytosis. Besides being a binding partner for integrin beta(3), a receptor known to mediate the uptake of apoptotic cells via activating Rac1, we also show that TG2 binds MFG-E8 (milk fat globulin EGF factor 8), a protein known to bridge integrin beta(3), to apoptotic cells. Finally, we report that in wild-type macrophages one or two engulfing portals are formed during phagocytosis of apoptotic cells that are characterized by accumulation of integrin beta(3) and Rac1. In the absence of TG2, integrin beta(3), cannot properly recognize the apoptotic cells, is not accumulated in the phagocytic cup, and its signaling is impaired. As a result, the formation of the engulfing portals, as well as the portals formed, is much less efficient. We propose that TG2 has a novel function to stabilize efficient phagocytic portals. The Journal of Immunology, 2009, 182: 2084-2092.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: Q Science > QP Physiology
Q Science > QR Microbiology > QR180 Immunology
Publisher: American Association of Immunologists, Inc.
ISSN: 0022-1767
Last Modified: 04 Jun 2017 03:04
URI: http://orca.cf.ac.uk/id/eprint/16100

Citation Data

Cited 63 times in Google Scholar. View in Google Scholar

Cited 86 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 55 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item