Tracking the host response to infection in peritoneal models of acute resolving inflammation

Millrine, David ORCID: https://orcid.org/0000-0002-8041-0151, Rice, Christopher M., Fernandez, Javier U. and Jones, Simon A. ORCID: https://orcid.org/0000-0001-7297-9711 2023. Tracking the host response to infection in peritoneal models of acute resolving inflammation. Jenkins, B.J., ed. Inflammation and Cancer, Vol. 2691. Methods in Molecular Biology, New York: Springer, pp. 81-95. (10.1007/978-1-0716-3331-1_7)

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Abstract

Antimicrobial host defense is dependent on the rapid recruitment of inflammatory cells to the site of infection, the elimination of invading pathogens, and the efficient resolution of inflammation that minimizes damage to the host. The peritoneal cavity provides an accessible and physiologically relevant system where the delicate balance of these processes may be studied. Here, we describe murine models of peritoneal inflammation that enable studies of competent antimicrobial immunity and inflammation-associated tissue damage as a consequence of recurrent bacterial challenge. The inflammatory hallmarks of these models reflect the clinical and molecular features of peritonitis seen in renal failure patients on peritoneal dialysis. The development of these models relies on the preparation of a cell-free supernatant derived from an isolate of Staphylococcus epidermidis (termed SES). Intraperitoneal administration of SES induces a Toll-like receptor 2-driven acute inflammatory response that is characterized by an initial transient influx of neutrophils that are replaced by a more sustained recruitment of mononuclear cells and lymphocytes. Adaptation of this model using a repeated administration of SES allows investigations into the development of adaptive immunity and the hallmarks associated with tissue remodelling and fibrosis. These models are therefore clinically relevant and provide exciting opportunities to study innate and adaptive immunity and the response of the stromal tissue compartment to bacterial infection and the ensuing inflammatory reaction.

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Springer
ISBN:	978-1-0716-3330-4
ISSN:	1064-3745
Date of First Compliant Deposit:	18 August 2023
Date of Acceptance:	24 June 2023
Last Modified:	22 Sep 2023 01:30
URI:	https://orca.cardiff.ac.uk/id/eprint/161922

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