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Triple helix formation of procollagen type I can occur at the rough endoplasmic reticulum membrane

Beck, Konrad ORCID: https://orcid.org/0000-0001-5098-9484, Boswell, B. A., Ridgway, C. C. and Bächinger, H. P. 1996. Triple helix formation of procollagen type I can occur at the rough endoplasmic reticulum membrane. Journal of Biological Chemistry 271 (35) , pp. 21566-21573. 10.1074/jbc.271.35.21566

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Abstract

One key problem in understanding the biosynthesis of collagens remains the assembly of the three alpha-chains. How and when are the different gene products selected, aligned, and folded into a triple helix? As the spatial arrangement during biosynthesis might be important, we concentrated on whether the rough endoplasmic reticular membrane is involved in this process. Microsomes were prepared from biosynthetically labeled chick tendon fibroblasts. Vesicles were spread as a monomolecular film which was then transferred over several compartments of a filmbalance containing fresh subphase. Fluorograms of the surface film showed that the monolayer contains procollagen chains. When the monolayer was transferred onto a chymotrypsin/trypsin-containing subphase, the gel bands of the proalpha-chains were shifted into the position of mature alpha-chains, indicating that only the propeptides were digested and the collagenous regions were protected due to triple helix formation. Our results suggest that newly synthesized proalpha-chains can associate as trimers and fold into a triple helical conformation while they are still associated with the membranes of the rough endoplasmic reticulum. These processes also occur when interchain disulfide linkage is inhibited, indicating that chain selection and registration is not dependent on formation of covalent bonds among the carboxyl propeptides.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: Q Science > QD Chemistry
Publisher: American Society for Biochemistry and Molecular Biology
ISSN: 0021-9258
Date of First Compliant Deposit: 7 June 2016
Last Modified: 05 Jan 2024 08:12
URI: https://orca.cardiff.ac.uk/id/eprint/16308

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