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Carbohydrate and amino acid metabolism of Spironucleus vortens

Millet, Coralie Ode, Lloyd, David, Coogan, Michael Peter, Randall, Joanna and Cable, Joanne 2011. Carbohydrate and amino acid metabolism of Spironucleus vortens. Experimental Parasitology 129 (1) , pp. 17-26. 10.1016/j.exppara.2011.05.025

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Abstract

The metabolism of Spironucleus vortens, a parasitic, diplomonad flagellate related to Giardia intestinalis, was investigated using a combination of membrane inlet mass spectrometry, (1)H NMR, (13)C NMR, bioscreen continuous growth monitoring, and ion exchange chromatography. The products of glucose-fuelled and endogenous metabolism were identified by (1)H NMR and (13)C NMR as ethanol, acetate, alanine and lactate. Mass spectrometric monitoring of gas metabolism in buffered cell suspensions showed that glucose and ethanol could be used by S. vortens as energy-generating substrates, but bioscreen automated monitoring of growth in culture medium, as well as NMR analyses, suggested that neither of these compounds are the substrates of choice for this organism. Ion-exchange chromatographic analyses of free amino-acid and amino-acid hydrolysate of growth medium revealed that, despite the availability of large pools of free amino-acids in the medium, S. vortens hydrolysed large amounts of proteins during growth. The organism produced alanine and aspartate, and utilised lysine, arginine, leucine, cysteine and urea. However, mass spectrometric and bioscreen investigations showed that addition of the utilised amino acids to diluted culture medium did not induce any significant increase in metabolic or growth rates. Moreover, as no significant amounts of ornithine were produced, and addition of arginine under aerobic conditions did not generate NO production, there was no evidence of the presence of an energy-generating, arginine dihydrolase pathway in S. vortens under in vitro conditions.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Chemistry
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Diplomonad; Spironucleus; Carbohydrate; Amino acid; Mass spectrometry; NMR
Publisher: Elsevier
ISSN: 0014-4894
Last Modified: 10 Oct 2017 13:34
URI: http://orca.cf.ac.uk/id/eprint/16335

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