Failure to apply standard limit-of-detection or limit-of-quantitation criteria to specialized pro-resolving mediator analysis incorrectly characterizes their presence in biological samples

O’Donnell, Valerie B. ORCID: https://orcid.org/0000-0003-4089-8460, Schebb, Nils H., Milne, Ginger L., Murphy, Michael P., Thomas, Christopher P. ORCID: https://orcid.org/0000-0001-5840-8613, Steinhilber, Dieter, Gelhaus, Stacy L., Kühn, Hartmut, Gelb, Michael H., Jakobsson, Per-Johan, Blair, Ian A., Murphy, Robert C., Freeman, Bruce A., Brash, Alan R. and FitzGerald, Garret A. 2023. Failure to apply standard limit-of-detection or limit-of-quantitation criteria to specialized pro-resolving mediator analysis incorrectly characterizes their presence in biological samples. Nature Communications 14 (1) , 7172. 10.1038/s41467-023-41766-w

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Abstract

Specialized pro-resolving mediators (SPM) derived from oxygenation of long chain polyunsaturated fatty acids (PUFA) were originally described by Serhan and colleagues and have been proposed as mediators of inflammation resolution. Families of SPM described in the literature include lipoxins, resolvins, maresins, protectins and their peptide conjugates. Gomez and co-authors reported that levels of plasma SPM from patients with early rheumatoid arthritis predict response to biologic therapy after 6 months. SPM were measured in this study using liquid chromatography tandem mass spectrometry (LC-MS/MS). On reviewing the methods, supplementary analytical data, and the online peer review file, we note serious concerns, regarding both analytical methods and experimental conclusions. Application of this flawed methodology to SPM analysis brings into question the very occurrence of many of these lipids in biological samples, their proposed impact on inflammatory processes, and claims of their utility as biomarkers.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	License information from Publisher: LICENSE 1: URL: http://creativecommons.org/licenses/by/4.0/, Type: open-access
Publisher:	Nature Research
Date of First Compliant Deposit:	10 November 2023
Date of Acceptance:	27 August 2023
Last Modified:	29 Nov 2023 09:07
URI:	https://orca.cardiff.ac.uk/id/eprint/163785

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