Adenine methylation is very scarce in the Drosophila genome and not erased by the ten-eleven translocation dioxygenase

Boulet, Manon, Gilbert, Guerric, Renaud, Yoan, Schmidt-Dengler, Martina, Plantié, Emilie, Bertrand, Romane, Nan, Xinsheng ORCID: https://orcid.org/0000-0002-0865-7934, Jurkowski, Tomasz ORCID: https://orcid.org/0000-0002-2012-0240, Helm, Mark, Vandel, Laurence and Waltzer, Lucas 2023. Adenine methylation is very scarce in the Drosophila genome and not erased by the ten-eleven translocation dioxygenase. eLife 12 , RP91655. 10.7554/elife.91655

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Abstract

N6-methyladenine (6mA) DNA modification has recently been described in metazoans, including in Drosophila, for which the erasure of this epigenetic mark has been ascribed to the ten-eleven translocation (TET) enzyme. Here, we re-evaluated 6mA presence and TET impact on the Drosophila genome. Using axenic or conventional breeding conditions, we found traces of 6mA by LC-MS/MS and no significant increase in 6mA levels in the absence of TET, suggesting that this modification is present at very low levels in the Drosophila genome but not regulated by TET. Consistent with this latter hypothesis, further molecular and genetic analyses showed that TET does not demethylate 6mA but acts essentially in an enzymatic-independent manner. Our results call for further caution concerning the role and regulation of 6mA DNA modification in metazoans and underline the importance of TET non-enzymatic activity for fly development.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Pharmacy Biosciences
Additional Information:	License information from Publisher: LICENSE 1: URL: http://creativecommons.org/licenses/by/4.0/
Publisher:	eLife Sciences Publications
Date of First Compliant Deposit:	2 January 2024
Last Modified:	02 Jan 2024 15:30
URI:	https://orca.cardiff.ac.uk/id/eprint/165141

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