Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Techniques for extraction of depth-resolved in vivo human retinal intrinsic optical signals with optical coherence tomography

Tumlinson, Alexandre Rex, Hermann, Boris, Hofer, Bernd, Povazay, Boris, Margrain, Thomas Hengist, Binns, Alison and Drexler, Wolfgang 2009. Techniques for extraction of depth-resolved in vivo human retinal intrinsic optical signals with optical coherence tomography. Japanese Journal of Ophthalmology 53 (4) , pp. 315-326. 10.1007/s10384-009-0684-5

Full text not available from this repository.

Abstract

Purpose To demonstrate acquisition and analysis methods for depth-resolved observation of slow retinal physiology induced changes in infrared backscatter in vivo. Methods A dark-adapted human was briefly subjected to a localized photobleach. For 20 min before and 30 min after the stimulus, volumetric optical coherence tomograms were collected partially overlapping the bleached region. Tomograms were segmented into retinal layers by a newly described algorithm exploiting information in adjacent B-scans. En face fundus images extracted from major intraretinal layers were laterally registered manually. Time series summarizing the observed backscatter in selected layers for the bleached and unbleached areas are shown with a variety of corrections and normalizations applied: tomograms were corrected for inherent sensitivity roll-off, and the ratio between other layers and an assumed unchanging layer (retinal pigment epithelium), as well as the ratio of the stimulated area to the unstimulated area, were calculated. Results Adjacent B-scan information allows a simpler segmentation algorithm to be used. Sensitivity roll-off correction reduces signal variability due to eye motion. After normalizations, the signal correlated with the stimulus appears strongest at the photoreceptor inner-outer segment junction. Conclusions Demonstrated methods manage data complexity and reduce uncorrelated signal variability. This single trial warrants further investigation of intrinsic optical signals to observe slow physiologic responses.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: functional - intrinsic optical signal - optical coherence tomography (OCT) - optophysiology - segmentation
Publisher: Springer
ISSN: 0021-5155
Last Modified: 05 Jan 2018 22:03
URI: http://orca.cf.ac.uk/id/eprint/17381

Citation Data

Cited 15 times in Google Scholar. View in Google Scholar

Cited 9 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item