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A new anti-loxoscelic serum produced against recombinant sphingomyelinase D: results of preclinical trials

de Almeida, Daniel Manzoni, Fernandes-Pedrosa, Matheus de F., de Andrade, Rute M. Goncalves, Marcelino, Jose Roberto, Gondo-Higashi, Hisako, de Azevedo, Inacio de L. M. Junqueira, Lee Ho, Paulo, Van Den Berg, Carmen Wilma and Tambourgi, Denise V. 2008. A new anti-loxoscelic serum produced against recombinant sphingomyelinase D: results of preclinical trials. American Journal of Tropical Medicine and Hygiene 79 (3) , pp. 463-470.

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Abstract

Envenomation by Loxosceles species (brown spider) can lead to local dermonecrosis and to serious systemic effects. The main toxic component in the venom of these spiders is sphingomyelinase D (SMase D) and various isoforms of this toxin are present in Loxosceles venoms. We have produced a new anti-loxoscelic serum by immunizing horses with recombinant SMase D. In the present study, we compared the neutralization efficacy of the new anti-loxoscelic serum and anti-arachnidic serum (the latter serum is used for therapy for loxoscelism in Brazil) against the toxic effects of venoms from spiders of the genus Loxosceles. Neutralization tests showed that anti-SMase D serum has a higher activity against toxic effects of L. intermedia and L. laeta venoms and similar or slightly weaker activity against toxic effects of L. gaucho than that of Arachnidic serum. These results demonstrate that recombinant SMase D can replace venom for anti-venom production and therapy.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RM Therapeutics. Pharmacology
Publisher: American Society of Tropical Medicine and Hygiene
ISSN: 0002-9637
Last Modified: 04 Jun 2017 03:10
URI: http://orca.cf.ac.uk/id/eprint/17565

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