Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Fibroblast growth factor 2 and transforming growth factor β1 induce precocious maturation of articular cartilage

Khan, Ilyas Mahmood, Evans, Samuel Lewin, Young, Robert David, Blain, Emma Jane, Quantock, Andrew James, Avery, Nick and Archer, Charles William 2011. Fibroblast growth factor 2 and transforming growth factor β1 induce precocious maturation of articular cartilage. Arthritis & Rheumatism 63 (11) , pp. 3417-3427. 10.1002/art.30543

Full text not available from this repository.

Abstract

Objective: We have discovered that a combination of fibroblast growth factor 2 and transforming growth factor β1 induce profound morphologic changes in immature articular cartilage. The purpose of this study was to test the hypothesis that these changes represent accelerated postnatal maturation. Methods: Histochemical and biochemical assays were used to confirm the nature of the morphologic changes that accompany growth factor stimulation of immature bovine articular cartilage explants in serum-free culture medium. Growth factor–induced apoptosis, cellular proliferation, and changes in the collagen network were also quantitatively analyzed. Results: Growth factor stimulation resulted in rapid resorption from the basal aspect of immature cartilage explants that was simultaneously opposed by cellular proliferation from the apical aspect driven from a pool of chondroprogenitor cells we have previously described. Maturation-dependent changes in tissue stiffness, collagen crosslinking, and collagen fibril architecture as well as differentiation of the extracellular matrix into distinct pericellular, territorial, and interterritorial domains were all present in growth factor–stimulated cartilage samples and absent in control samples. Conclusion: Our data demonstrate that it is possible to significantly enhance the maturation of cartilage tissue using specific growth factor stimulation. This may have applications in transplantation therapy or in the treatment of diseased cartilage, through phenotype modulation of osteoarthritic chondrocytes in order to stimulate growth and maturation of cartilage repair tissue.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Engineering
Biosciences
Subjects: Q Science > QP Physiology
Publisher: John Wiley & Sons
ISSN: 0004-3591
Last Modified: 06 Mar 2018 20:52
URI: http://orca.cf.ac.uk/id/eprint/18393

Citation Data

Cited 16 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 15 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item