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PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)

Brede, Edward G., Long, Peter, Zefania, Sama, Rabenandrasana, Marc, Székely, Tamás and Bruford, Michael William 2010. PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri). Conservation Genetics Resources 2 (Supp 1) , pp. 273-277. 10.1007/s12686-010-9189-2

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Abstract

We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species’ range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean H e = 0.544 (range 0.0678–0.885), mean H o = 0.430 (range 0.0400–0.767) and mean N a = 4.59 (range 2–10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Sustainable Places Research Institute (PLACES)
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Uncontrolled Keywords: Amaurornis olivieri Canirallus kioloides; Rail; Waterbird; Madagascar; Microsatellite; Primer; Endangered
Publisher: Springer
ISSN: 1877-7252
Last Modified: 04 Jun 2017 03:18
URI: http://orca.cf.ac.uk/id/eprint/19481

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