Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Wake-up-call, a lin-52 paralogue, and always early, a lin-9 homologue physically interact, but have opposing functions in regulating testis-specific gene expression

Doggett, Karen, Jiang, Jianqiao, Aleti, Gajender and White-Cooper, Helen 2011. Wake-up-call, a lin-52 paralogue, and always early, a lin-9 homologue physically interact, but have opposing functions in regulating testis-specific gene expression. Developmental Biology 355 (2) , pp. 381-393. 10.1016/j.ydbio.2011.04.030

Full text not available from this repository.

Abstract

A conserved multi-subunit complex (MybMuvB, MMB), regulates transcriptional activity of many different target genes in Drosophila somatic cells. A paralogous complex, tMAC, controls expression of at least 1500 genes in the male germline, and is essential for sperm production. The roles of specific subunits of tMAC, MMB or orthologous complexes in regulating target gene expression are not understood. MMB and orthologous complexes have Lin-52 as a subunit, but Lin-52 did not co-purify with tMAC. We identified wake-up-call (wuc), a lin-52 paralogue, via a physical interaction with the tMAC lin-9-related subunit Aly, and find that Wuc co-localises with known tMAC subunits. We show that wuc, like aly, is required for spermatogenesis. However, despite phenotypic similarities, the role of wuc is very different from that of previously characterised tMAC mutants. Unlike aly, loss of wuc results in only relatively mild defects in testis-specific gene expression. Strikingly, wuc loss of function partially rescues expression of target genes in aly mutant testes. We propose that wuc represses testis-specific gene expression, that this repression is counteracted by aly, and that aly and a testis-specific TFIID complex work together to promote high transcriptional activity of spermiogenic genes specifically in primary spermatocytes.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: testis gene expression; male fertility; Drosophila; transcription
Publisher: Elsevier
ISSN: 0012-1606
Last Modified: 04 Jun 2017 03:28
URI: http://orca.cf.ac.uk/id/eprint/21719

Citation Data

Cited 19 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 10 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item