Uptake and localisation of rhenium fac-tricarbonyl polypyridyls in fluorescent cell imaging experiments

Fernández-Moreira, Vanesa ORCID: https://orcid.org/0000-0002-1218-7218, Thorp-Greenwood, Flora Lottie, Amoroso, Angelo James ORCID: https://orcid.org/0000-0002-7601-5482, Cable, Joanne ORCID: https://orcid.org/0000-0002-8510-7055, Court, Jonathan B., Gray, Victoria, Hayes, Anthony Joseph, Jenkins, Robert Leyshon, Kariuki, Benson ORCID: https://orcid.org/0000-0002-8658-3897, Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571, Millet, Coralie Ode, Williams, Catrin Ffion ORCID: https://orcid.org/0000-0001-8619-2581 and Coogan, Michael Peter 2010. Uptake and localisation of rhenium fac-tricarbonyl polypyridyls in fluorescent cell imaging experiments. Organic and Biomolecular Chemistry 8 (17) , pp. 3888-3901. 10.1039/c004610h

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Abstract

The synthesis of a series of rhenium fac tricarbonyl bisimine complexes and their application as lumophores in fluorescence imaging of yeast and human adenocarcinoma cells is reported. A wide range of complexes are synthesised with varying charges and lipophilicities, all of which have photophysical properties which make them suitable as cell imaging agents. After attempts to apply these as imaging agents in various strains of yeast which showed limited uptake, an investigation was undertaken of their applications as imaging agents in mammalian cells. In general the uptake was high and short-term toxicity and photobleaching appear to be low. The patterns of uptake and localisation are correlated with structural and electronic features of the complexes in an attempt to establish ground-rules for the design and application of rhenium complexes in imaging of eukaryotes.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Chemistry
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Publisher:	Royal Society of Chemistry
ISSN:	1477-0520
Last Modified:	05 Nov 2022 15:04
URI:	https://orca.cardiff.ac.uk/id/eprint/22273

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