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NCAM and PSA-NCAM dependent membrane spreading and F-actin reorganization in suspended adhering neural cells

Bazou, Despina, Blain, Emma Jane and Terence Coakley, W. 2008. NCAM and PSA-NCAM dependent membrane spreading and F-actin reorganization in suspended adhering neural cells. Molecular Membrane Biology 25 (2) , pp. 102-114. 10.1080/09687680701618365

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Abstract

Mediation of synchronous cell-cell interactions by NCAM and PSA-NCAM is examined here in aggregates (monolayers) of C6 polysialylated embryonic neural cells, formed rapidly (within 30 s) in suspension in an ultrasound trap. These cells express all three main isoforms of neural cell adhesion molecule (NCAM). The rate of extension of perimeter contact (i.e., membrane spreading) between closely adjacent cells and the temporal reinforcement of the Filamentous (F)-actin cytoskeleton at those regions were measured. Enzymatic removal of the cell-cell repelling polysialic acid (PSA) increases the rate of NCAM-induced membrane spreading, while removal of NCAM-120 had no detectable effect. Competitive peptide inhibition of the third immunoglobulin domain of NCAM significantly reduced the rate of membrane spreading, while NCAM siRNA transfected cells lost their ability to spread. It is argued that NCAM induced contact is the initial requirement for membrane spreading and facilitates conditions for subsequent cytoskeletal reorganization in these neural cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: cell adhesion; actin; physical cell trap; siRN; peptide
Publisher: Informa Healthcare
ISSN: 0968-7688
Last Modified: 29 Apr 2019 09:54
URI: http://orca.cf.ac.uk/id/eprint/22617

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