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Telomere dynamics during replicative senescence are not directly modulated by conditions of oxidative stress in IMR90 fibroblast cells

Britt-Compton, Bethan, Wyllie, Fiona Sandra, Rowson, Janet Maud, Capper, Rebecca, Jones, Rhiannon E. and Baird, Duncan Martin 2009. Telomere dynamics during replicative senescence are not directly modulated by conditions of oxidative stress in IMR90 fibroblast cells. Biogerontology 10 (6) , pp. 683-693. 10.1007/s10522-009-9216-4

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Abstract

The replicative lifespan of many cell types is determined by the length of telomeres in the initiating cell population. In 20% oxygen, IMR90 cells have a shorter replicative lifespan compared to that achieved in conditions that lower the levels of oxidative stress. We sought to address the role of telomere dynamics in determining the replicative lifespan of IMR90 cells. We analysed clonal populations cultured in parallel in 3 and 20% oxygen. We observed that, at senescence, telomere length was shorter in 3% oxygen and this was proportional to the lifespan extension. We observed no detectable difference in the rate of telomere erosion in the two culture conditions, however as the cells approached senescence the growth rate of the cultures slowed with a commensurate increase in the rate of telomere erosion. We conclude that, in 20% oxygen senescence of IMR90 is telomere-independent, but telomere-dependent in 3% oxygen.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Medicine
Subjects: R Medicine > R Medicine (General)
R Medicine > RB Pathology
Uncontrolled Keywords: Telomere; Telomerase; Senescence; Oxidative stress; Reacive oxygen species; replicative lifespan
Publisher: Springer
ISSN: 1389-5729
Last Modified: 06 Jun 2019 20:40
URI: http://orca.cf.ac.uk/id/eprint/23234

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