Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Analysis of human tumour necrosis factor receptor 1 dominant-negative mutants reveals a major region controlling cell surface expression

Fielding, Ceri Alan, Siebert, Stefan, Rowe, Martin and Brennan, Paul 2004. Analysis of human tumour necrosis factor receptor 1 dominant-negative mutants reveals a major region controlling cell surface expression. FEBS Letters 570 (1-3) , pp. 138-142. 10.1016/j.febslet.2004.06.035

Full text not available from this repository.

Abstract

Tumour necrosis factor receptor 1 (TNFR1) plays a critical role in host defence and inflammation. We have identified a membrane proximal region (aa 218-324) of TNFR1 that restricts surface expression. This was prompted by comparing the dominant-negative properties of a C-terminal truncation of TNFR1 with a point mutant that prevents signalling. C-terminal truncation (aa 218-426) generates a better dominant-negative TNFR1 mutant than inactivation of the death domain by point mutation. The increased dominant-negative activity correlates with increased cell surface expression. The membrane proximal region is the most important region of the receptor for restricting expression.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Uncontrolled Keywords: Tumour necrosis factor receptor 1; Nuclear factor-κB; Structure–function; Dominant-negative receptor; Surface expression
Publisher: Elsevier
ISSN: 0014-5793
Last Modified: 04 Jun 2017 01:31
URI: http://orca.cf.ac.uk/id/eprint/233

Citation Data

Cited 6 times in Google Scholar. View in Google Scholar

Cited 6 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item