Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Resveratrol, but not dihydroresveratrol, induces premature senescence in primary human fibroblasts

Faragher, Richard G. A., Burton, Dominic G. A., Majecha, Patricia, Fong, Noel S. Y., Davis, Terence ORCID: https://orcid.org/0000-0003-2780-0262, Sheerin, Angela and Ostler, Elizabeth L. 2011. Resveratrol, but not dihydroresveratrol, induces premature senescence in primary human fibroblasts. AGE 33 (4) , pp. 555-564. 10.1007/s11357-010-9201-5

Full text not available from this repository.

Abstract

Resveratrol, trans-3,5,4′-trihydroxystilbene, is a polyphenolic compound which has been reported to mimic the gene expression patterns seen in whole animals undergoing dietary restriction. The mechanism of action of resveratrol remains poorly understood, but modulation of both cellular proliferation and apoptosis has been proposed as important routes by which the molecule may exert its effects. This study reports the effects of both resveratrol and dihydroresveratrol (a primary in vivo metabolite) on the proliferative capacity of human primary fibroblasts. No generalised reduction in the growth fraction was observed when fibroblasts derived from three different tissues were treated with resveratrol at concentrations of 10 μm or less. However, concentrations above 25 μm produced a dose-dependent reduction in proliferation. This loss of the growth fraction was paralleled by an increase in the senescent fraction as determined by staining for senescence associated beta galactosidase and dose recovery studies conducted over a 7-day period. Entry into senescence in response to treatment with resveratrol could be blocked by a 30-min preincubation with the p38 MAP kinase inhibitor SB203580. No effects on proliferation were observed when cells were treated with dihydroresveratrol at concentrations of up to 100 μm.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Resveratrol – Cellular senescence – Ageing – MRC5 – HCA2 – Ek1.Br – p38 MAP kinase
Publisher: Springer
ISSN: 0161-9152
Last Modified: 14 Dec 2022 02:14
URI: https://orca.cardiff.ac.uk/id/eprint/23609

Citation Data

Cited 18 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item