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An investigation on the effect of the supernatant of porphyromonas gingivalis exposed to titanium on an ex vivo mandible culture model

Sakellariou, Nikolaos 2011. An investigation on the effect of the supernatant of porphyromonas gingivalis exposed to titanium on an ex vivo mandible culture model. MScD Thesis, Cardiff University.
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Oral Rehabilitation with titanium implants has become one of the most promising treatment modalities in dentistry. Although treatment with titanium implants is highly successful and predictable, it has been identified that chronic inflammatory diseases associated with bacterial colonisation, occur around the implant fixtures and may affect the supporting tissues of dental implants and their long-term success. Porphyromonas gingivalis, a Gram-negative anaerobe, is one of the prime pathogens identified as implicated in the development of both these diseases. An ex vivo culture model of 28-days old wistar rats mandible slices has been utilised to investigate the local response of the tissues to the supernatant produced by Porphyromonas gingivalis. To study the effect of titanium on virulence, three strains of P. gingivalis were cultured for 48 hours in the presence of smoothened and moderately roughened titanium specimens prior to supernatant collection. The ex vivo culture model was then exposed for a total of either 3 or 7 days to concentrations of 50μg/ml and 100μg/ml of supernatants from the three strains of P. gingivalis grown in the different conditions. The supernatants, which contain the extracellular produce of P. gingivalis, had an adverse effect in the periodontal connective tissues (ligament) of the mandible slices of this model, in that it caused extensive cell death and disorganisation of the matrix of these tissues and specific cellular responses. However, the presence of titanium (either smoothened or moderately roughened),whilst producing the supernatant did not appear to have an important additional effect on virulence in this model.

Item Type: Thesis (MScD)
Status: Unpublished
Schools: Dentistry
Subjects: Q Science > Q Science (General)
R Medicine > RK Dentistry
Date of First Compliant Deposit: 30 March 2016
Last Modified: 19 Mar 2016 22:41

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