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Differential relocation and stability of PML-body components during productive human cytomegalovirus infection: Detailed characterization by live-cell imaging

Dimitropoulou, Panagiota, Caswell, Richard Charles, McSharry, Brian P., Greaves, Richard F., Spandidos, Demetrios A., Wilkinson, Gavin William Grahame and Sourvinos, George 2010. Differential relocation and stability of PML-body components during productive human cytomegalovirus infection: Detailed characterization by live-cell imaging. European Journal of Cell Biology 89 (10) , pp. 757-768. 10.1016/j.ejcb.2010.05.006

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Abstract

In controlling the switch from latency to lytic infection, the immediate early (IE) genes lie at the core of herpesvirus pathogenesis. To image the 72 kDa human cytomegalovirus (HCMV) major IE protein (IE1-72K), a recombinant virus encoding IE1 fused with EGFP was constructed. Using this construct, the IE1-EGFP fusion was detected at ND10 (PML-bodies) within 2 h post infection (p.i.) and the complete disruption of ND10 imaged through to 6 h p.i. HCMV genomes and IE2-86K protein could be detected adjacent to the slowly degrading IE1-72K/ND10 foci. IE1-72K associates with metaphase chromatin, recruiting both PML and STAT2. hDaxx, STAT1 and IE2-86K did not re-locate to metaphase chromatin; the fate of hDaxx is particularly important as this protein contributes to an intrinsic barrier to HCMV infection. While IE1-72K participates in a complex with chromatin, PML, STAT2 and Sp100, IE1-72K releases hDaxx from ND10 yet does not appear to remain associated with it.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Medicine
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > QR Microbiology > QR355 Virology
R Medicine > R Medicine (General)
Uncontrolled Keywords: HCMV; IE1-72K; ND10; PML; Sp100; hDaxx; STAT1; STAT2; Condensed chromatin; Live-cell microscopy
Publisher: Elsevier
ISSN: 0171-9335
Last Modified: 07 Mar 2019 21:53
URI: http://orca.cf.ac.uk/id/eprint/24758

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