Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

A transcriptomic analysis of the EK1.Br strain of human fibroblastoid keratocytes: the effects of growth, quiescence and senescence

Kipling, David Glyn, Jones, Dawn L., Smith, Susan Kaye, Giles, Peter James ORCID: https://orcid.org/0000-0003-3143-6854, Jennert-Burston, Katrin, Ibrahim, Badr, Sheerin, Angela N.P., Evans, Amy J. C., Rhys-Willams, William and Faragher, Richard G.A. 2009. A transcriptomic analysis of the EK1.Br strain of human fibroblastoid keratocytes: the effects of growth, quiescence and senescence. Experimental Eye Research 88 (2) , pp. 277-285. 10.1016/j.exer.2008.11.030

Full text not available from this repository.

Abstract

There is a growing need within ocular research for well-defined cellular models of normal corneal biology. To meet this need we created and partially characterised a standard strain of human fibroblastoid keratocytes (EK1.Br) and demonstrated that phenotypic changes occur within these cells with replicative senescence in vitro. Using Affymetrix HG-U133A oligonucleotide arrays, this paper reports both a comprehensive analysis of the transcriptome of EK1.Br in the growing, quiescent and senescent states and a comparison of that transcriptome with those of primary corneal endothelium, lung fibroblasts and dermal fibroblasts grown under identical conditions. Data mining shows (i) that EK1.Br retain the characteristic transcriptional fingerprint of keratocytes in vitro (ii) that this phenotype can be distinguished from those of other ‘fibroblasts’ by groups of highly differentially expressed genes and (iii) that senescence induces a distinct dedifferentiation phenomenon in EK1.Br. These findings are contextualised into the broader literature on replicative senescence and are supported with a web-accessible and fully searchable public-access database (www.madras.cf.ac.uk/cornea).

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RB Pathology
Uncontrolled Keywords: senescence, gene expression, keratocyte, fibroblast, cornea
Publisher: Elsevier
ISSN: 0014-4835
Last Modified: 05 Nov 2022 16:12
URI: https://orca.cardiff.ac.uk/id/eprint/25088

Citation Data

Cited 17 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item