Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Extralymphoid CD8+ T cells resident in tissue from simian immunodeficiency virus SIVmac239 nef-vaccinated macaques suppress SIVmac239 replication ex vivo

Greene, Justin M., Lhost, Jennifer J., Burwitz, Benjamin J., Budde, Melisa L., Macnair, Caitlin E., Weiker, Madelyn K., Gostick, Emma, Friedrich, Thomas C., Broman, Karl W., Price, David, O'Connor, Shelby L. and O'Connor, David H. 2010. Extralymphoid CD8+ T cells resident in tissue from simian immunodeficiency virus SIVmac239 nef-vaccinated macaques suppress SIVmac239 replication ex vivo. Journal of Virology 84 (7) , pp. 3362-3372. 10.1128/JVI.02028-09

[img]
Preview
PDF - Published Version
Download (3MB) | Preview

Abstract

Live-attenuated vaccination with simian immunodeficiency virus (SIV) SIVmac239Δnef is the most successful vaccine product tested to date in macaques. However, the mechanisms that explain the efficacy of this vaccine remain largely unknown. We utilized an ex vivo viral suppression assay to assess the quality of the immune response in SIVmac239Δnef-immunized animals. Using major histocompatibility complex-matched Mauritian cynomolgus macaques, we did not detect SIV-specific functional immune responses in the blood by gamma interferon (IFN-γ) enzyme-linked immunospot assay at select time points; however, we found that lung CD8+ T cells, unlike blood CD8+ T cells, effectively suppress virus replication by up to 80%. These results suggest that SIVmac239Δnef may be an effective vaccine because it elicits functional immunity at mucosal sites. Moreover, these results underscore the limitations of relying on immunological measurements from peripheral blood lymphocytes in studies of protective immunity to HIV/SIV.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > QR Microbiology
Additional Information: Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0022-538X/ (accessed 25/02/2014)
Publisher: American Society for Microbiology
ISSN: 0022-538X
Date of First Compliant Deposit: 30 March 2016
Last Modified: 11 Sep 2017 13:01
URI: http://orca.cf.ac.uk/id/eprint/27491

Citation Data

Cited 26 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics