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Control of the proliferation of activated CD4+ T cells by connexins

Oviedo-Orta, Ernesto, Perreau, Matthieu, Evans, William Howard and Potolicchio, Ilaria 2010. Control of the proliferation of activated CD4+ T cells by connexins. Journal of Leukocyte Biology 88 (1) , pp. 79-86. 10.1189/jlb.0909613

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Abstract

As expression of Cxs in cells of the immune system increases upon cellular activation, we investigated whether Cxs and especially CxHcs play a major role during T cell-mediated responses. In particular, we studied the expression of Cx43Hc following CD4+ T cell stimulation using flow cytometry, real-time PCR, and Western blot analysis. We showed that expression of Cx43 and its phosphorylated isoforms increased in response to the engagement of CD3 and CD28. Cx43Hcs were found to be involved in sustaining proliferation of T cells, as assessed by cell cycle staining, thymidine incorporation assays, and CFSE analysis of cells exposed to mimetic peptide inhibitors of the plasma membrane Cx channels and antibodies generated to an extracellular region of Cx. The reduction of T cell proliferation mediated by Cx channel inhibitors suppressed cysteine uptake but not cytokine production. We conclude that upon antigen recognition, T cells require CxHc to sustain their clonal expansion.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology > QR180 Immunology
R Medicine > R Medicine (General)
Uncontrolled Keywords: Connexin 43 ; T cell receptor ; Hemichannels ; Cysteine uptake ; Cell cycle ; Lymphocytes ; Gap junction
Publisher: Society of Leukocyte Biology
ISSN: 0741-5400
Last Modified: 17 Mar 2021 02:50
URI: https://orca.cardiff.ac.uk/id/eprint/28436

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