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Growth differentiation factor-9 expression is inversely correlated with an aggressive behaviour in human bladder cancer cells

Du, Peng, Ye, Lin, Li, Han, Ruge, Fiona, Yang, Yong and Jiang, Wen Guo 2012. Growth differentiation factor-9 expression is inversely correlated with an aggressive behaviour in human bladder cancer cells. International Journal of Molecular Medicine 29 (3) , pp. 428-434. 10.3892/ijmm.2011.858

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Abstract

Growth differentiation factor-9 (GDF-9) is a family member of bone morphogenetic proteins (BMPs), which belong to the TGF-β superfamily. There has been a recent surge of interest in the role of growth differentiation factors and other BMPs in the development and spread of cancer. However, the role of GDF-9 in bladder cancer remains unknown. The present study investigated the expression of GDF-9 in normal and malignant human bladder tissue and its molecular interactions within bladder cancer cells. The expression of GDF-9 in human bladder tissues and bladder cancer cell lines was assessed at both the mRNA and protein levels using RT-PCR and immunohistochemistry, respectively. Full-length GDF-9 cDNA was amplified from normal mammary tissues. GDF-9 was overexpressed in bladder cancer cell lines using a mammalian expression construct. The effect of GDF-9 on cellular functions, was examined in bladder cancer cells overexpressing GDF-9 using a variety of in vitro assays. In normal bladder tissues, stronger staining of GDF-9 was seen in transitional cells, both in the cytoplasm and in the nucleus. In contrast, the staining of GDF-9 was notably weak or absent in cancer cells of tumour tissues. Similarly, the bladder cancer cell lines RT112 and EJ138, expressed very low levels of GDF-9. Moreover, overexpression of GDF-9 reduced the growth, adhesion and migration of bladder cell lines in vitro. However, the overexpression of GDF-9 had little bearing on the invasion of bladder cell lines in vitro. In conclusion, GDF-9 is expressed at lower levels in human bladder cancer cells compared with normal transitional cells of the bladder. GDF-9 levels are inversely correlated with the growth, adhesion and migration of bladder cancer cells in vitro. The results of the present study suggest that GDF-9 is a potential tumour suppressor in human bladder cancer.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Additional Information: Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/1107-3756/ (accessed 24/02/2014).
Publisher: Spandidos Publications
ISSN: 1107-3756
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 03:57
URI: http://orca.cf.ac.uk/id/eprint/29572

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