Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Differential regulation of chemokine production in human peritoneal mesothelial cells: IFN-gamma controls neutrophil migration across the mesothelium in vitro and in vivo

Robson, Rachel L., McLoughlin, Rachel Mary, Witowski, Janusz, Loetscher, Pius, Wilkinson, Thomas S., Jones, Simon Arnett and Topley, Nicholas 2001. Differential regulation of chemokine production in human peritoneal mesothelial cells: IFN-gamma controls neutrophil migration across the mesothelium in vitro and in vivo. The Journal of Immunology 167 (2) , pp. 1028-1038.

Full text not available from this repository.

Abstract

Leukocyte recruitment into the infected peritoneal cavity consists of an early, predominant polymorphonuclear leukocyte (PMN) influx and subsequent, prolonged mononuclear cell migration phase. Although chemokine secretion by resident peritoneal cells plays a primary role in mediating this migration, the mechanisms involved in controlling the switch in phenotype of cell infiltrate remain unclear. The present study investigates a potential role for the Th1-type cytokine IFN- in the process of leukocyte recruitment into the peritoneal cavity. Stimulation of cultured human peritoneal mesothelial cells with IFN- (1–100 U/ml) alone or in combination with IL-1 (100 pg/ml) or TNF- (1000 pg/ml) resulted in significant up-regulation of monocyte chemoattractant protein-1 and RANTES protein secretion. In contrast, IFN- inhibited basal and IL-1-, and TNF--induced production of IL-8. The modulating effects of IFN- on chemokine production occurred at the level of gene expression, and the degree of regulation observed was dependent on the doses of IL-1 and TNF- used. Analysis of the functional effects of IFN- on IL-1-induced transmesothelial PMN migration with an in vitro human transmigration system and an in vivo murine model of peritoneal inflammation demonstrated that IFN- was able to down-regulate PMN migration induced by optimal doses of IL-1. These effects were mediated in vivo via down-regulation of CXC chemokine synthesis. These findings suggest that IFN- may play a role in controlling the phenotype of infiltrating leukocyte during the course of an inflammatory response, in part via regulation of resident cell chemokine synthesis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Publisher: American Association of Immunologists
ISSN: 0022-1767
Last Modified: 30 Jun 2017 01:31
URI: http://orca.cf.ac.uk/id/eprint/297

Citation Data

Cited 56 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item