Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

A mechanism for TCR sharing between T cell subsets and individuals revealed by pyrosequencing

Venturi, Vanessa, Quigley, Maire F., Greenaway, Hui Yee, Ng, Pauline C., Ende, Zachary S., McIntosh, Tina, Asher, Tedi E., Almeida, Jorge R., Levy, Samuel, Price, David, Davenport, Miles P. and Douek, Daniel C. 2011. A mechanism for TCR sharing between T cell subsets and individuals revealed by pyrosequencing. The Journal of Immunology 186 (7) , pp. 4285-4294. 10.4049/jimmunol.1003898

Full text not available from this repository.

Abstract

The human naive T cell repertoire is the repository of a vast array of TCRs. However, the factors that shape their hierarchical distribution and relationship with the memory repertoire remain poorly understood. In this study, we used polychromatic flow cytometry to isolate highly pure memory and naive CD8+ T cells, stringently defined with multiple phenotypic markers, and used deep sequencing to characterize corresponding portions of their respective TCR repertoires from four individuals. The extent of interindividual TCR sharing and the overlap between the memory and naive compartments within individuals were determined by TCR clonotype frequencies, such that higher-frequency clonotypes were more commonly shared between compartments and individuals. TCR clonotype frequencies were, in turn, predicted by the efficiency of their production during V(D)J recombination. Thus, convergent recombination shapes the TCR repertoire of the memory and naive T cell pools, as well as their interrelationship within and between individuals.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > QR Microbiology > QR180 Immunology
Publisher: American Association of Immunologists
ISSN: 0022-1767
Last Modified: 04 Jun 2017 03:58
URI: http://orca.cf.ac.uk/id/eprint/29858

Citation Data

Cited 68 times in Google Scholar. View in Google Scholar

Cited 113 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item