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The transcript expression and protein distribution pattern in human colorectal carcinoma reveal a pivotal role of COM-1/p8 as a tumour suppressor

Davies, Mansel Leigh, Parr, Christian, Sanders, Andrew James, Fodstad, Oystein and Jiang, Wen Guo 2010. The transcript expression and protein distribution pattern in human colorectal carcinoma reveal a pivotal role of COM-1/p8 as a tumour suppressor. Cancer Genomics and Proteomics 7 (2) , pp. 75-80.

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Abstract

Background and Aims: COM-1(P8) is thought to play a role in the formation of metastases. This appears from current evidence to be different in various types of solid tumours. We aimed to examine the role COM-1 played in the development of colorectal cancer. Materials and Methods: The expression of COM-1 mRNA was examined using a quantitative polymerase chain reaction (PCR) technique together with immunohistochemistry to examine expression and distribution of the COM-1 protein in human colorectal carcinoma and matched normal colorectal mucosa. Results: COM-1 was expressed in 22.8% of normal colorectal mucosa samples and the expression in these tissues was 54.9 copies of COM-1 transcript per sample. In tumour tissues, 43.6% of samples expressed COM-1, at a level of 98.9 copies of COM-1 transcript per sample (p=0.012). Normal tissues demonstrated strong nuclear and peri-nuclear staining for COM-1 on immunohistochemistry (IHC) and in tumour tissues, the level of staining was found to be much greater, with a greater degree of cytoplasmic staining and little nuclear staining. Early-stage tumours showed a greater degree of staining on IHC compared to those at an advanced stage of disease. Conclusion: COM-1, although overexpressed at the messenger level, appears to be distributed in a cytoplasmic fashion at the protein level in tumours. Tumours at advanced stage express COM-1 protein to a lesser extent than their early-stage counterparts.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords: COM-1; p8; Colorectal carcinoma; Metastasis
Publisher: International Institute of Anticancer Research
ISSN: 1109-6535
Last Modified: 04 Jun 2017 04:00
URI: http://orca.cf.ac.uk/id/eprint/30467

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