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Oral mucosal progenitor cells are potently immunosuppressive in a dose-independent manner

Davies, Lindsay Catrina, Lönnies, Helena, Locke, Matthew, Sundberg, Berit, Rosendahl, Kerstin, Götherström, Cecilia, Le Blanc, Katarina and Stephens, Philip 2012. Oral mucosal progenitor cells are potently immunosuppressive in a dose-independent manner. Stem Cells and Development 21 (9) , pp. 1478-1487. 10.1089/scd.2011.0434

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Abstract

Oral mucosal lamina propria progenitor cells (OMLP-PCs) are a novel, clonally derived PC population of neural crest origin with the potential to differentiate down both mesenchymal and neuronal cell lineages. In this study we aimed to determine the immunological properties of OMLP-PCs and to establish whether they would be suitable candidates for allogeneic tissue engineering and in the treatment of immune-related diseases. OMLP-PCs demonstrated no inherent immunogenicity with insignificant expression of costimulatory molecules (CD40, CD80, CD86, CD154, and CD178) or human leukocyte antigen (HLA) class II. OMLP-PCs required 7 days of stimulation with interferon-γ (IFN-γ) to induce cell surface expression of HLA II. Mixed lymphocyte cultures and mitogen stimulation demonstrated the potent immunosuppressive capability of OMLP-PCs in a contact-independent manner. Complete inhibition of lymphocyte proliferation was seen at doses as low as 0.001% OMLP-PCs to responder lymphocytes, while annexin V staining confirmed that this immunosuppressive effect was not due to the induction of lymphocyte apoptosis. These data demonstrate, for the first time, that OMLP-PC immunomodulation, unlike that for mesenchymal stem cells, occurs via a dose- and HLA II–independent mechanism by the release of immunosuppressive soluble factors and suggests these cells may have wide ranging potential in future immune-related therapies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine > RK Dentistry
Publisher: Mary Ann Liebert
ISSN: 1547-3287
Funders: MRC
Last Modified: 04 Jun 2017 04:03
URI: http://orca.cf.ac.uk/id/eprint/31728

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