Sorting of human peripheral blood T-cell subsets using immunomagnetic beads

Wang, Edward Chung Yern ORCID: https://orcid.org/0000-0002-2243-4964 1998. Sorting of human peripheral blood T-cell subsets using immunomagnetic beads. Pound, John D., ed. Immunochemical Protocols, Vol. 80. Methods in Molecular Biology, vol. 2. Humana Press, pp. 365-376. (10.1007/978-1-59259-257-9_37)

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Abstract

Immunomagnetic beads are uniform, polymer particles coated with a polystyrene shell that provides both a smooth, hydrophobic surface to facilitate absorption of molecules, such as antibodies, and surface hydroxyl groups that allow covalent chemical binding of other bioreactive molecules, such as streptavidin, lectins, and peptides. Iron (III) oxide (Fe2O3) deposited in the core gives the beads superparamagnetic properties that lead to consistent and reproducible reactions to a magnetic field without permanent magnetization of the particles. These are the two qualities on which immunomagnetic separation (IMS) depends.

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Animals; Antibodies, Monoclonal; Mice; Humans; Cell Separation; Antibodies;Indicators and Reagents; Antigens, CD; CD8-Positive T-Lymphocytes; Immunoglobulin G;T-Lymphocyte Subsets; Sheep; Immunomagnetic Separation
Publisher:	Humana Press
ISBN:	9780896034938
ISSN:	1064-3745
Last Modified:	20 Oct 2022 09:24
URI:	https://orca.cardiff.ac.uk/id/eprint/31851

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