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Isolation of mouse mammary epithelial subpopulations: a comparison of leading methods

Smalley, Matthew John, Kendrick, Howard, Sheridan, Julie M., Regan, Joseph L., Prater, Michael D., Lindeman, Geoffrey J., Watson, Christine J., Visvader, Jane E. and Stingl, John 2012. Isolation of mouse mammary epithelial subpopulations: a comparison of leading methods. Journal of Mammary Gland Biology and Neoplasia 17 (2) , pp. 91-97. 10.1007/s10911-012-9257-1

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Abstract

Isolation of mammary epithelial subpopulations, including stem and progenitor cells, has become a standard technique in recent years. However, a number of methods and approaches for this have developed and the relative benefits of the different approaches, and the reason for their development, have not always been clear. Here, three of the leading laboratories working on the separation of mammary cell subpopulations have summarised their methods, highlighted their differences and similarities and also discussed the reasoning behind the approaches they have taken. This article will assist workers establishing mammary cell separation protocols in their laboratories to make informed choices about the methods they should use.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords: Mammary; Stem; Progenitor; Epithelial; Sorting; Isolation
Additional Information: Online first publication
Publisher: Springer
ISSN: 1083-3021
Last Modified: 04 Jun 2017 04:05
URI: http://orca.cf.ac.uk/id/eprint/32613

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Cited 27 times in Web of Science. View in Web of Science.

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